Cyp2a5 antibody
WebSep 1, 2000 · Expression of the CYP2A13 protein was confirmed by immunoblot analysis with an antibody to mouse CYP2A5 . As shown in the inset of Fig. 2, a single band of the expected size was detected in the microsomal fraction of CYP2A13-expressing Sf9 cells, but not in control cells. The recombinant CYP2A13 migrated to the same position as did a … WebWe've collected all of our primary antibody content in one place to help you easily find product information and resources. Here you will find application protocols and technical …
Cyp2a5 antibody
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WebNational Center for Biotechnology Information WebOct 23, 2024 · Primary antibodies like Anti-AP-2α Antibody (3B5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily …
WebImmunopurified proteins, pulled down with anti-CYP2A5 from 500 g of lung microsomes or 20 to ϳ 25 fmol of recombinant CYP2A6 or CYP2A13 (both in insect Sf9-cell microsomes), were analyzed on ... WebAnti-CYP2A5 Antibody. Mouse liver CYP2A5 protein was purified by octylsepharose and hydroxyapatite chromatography as previ-ouslydescribed.27 …
WebDec 8, 2024 · Cyp2a5 represents the first example of a P450 gene regulated by oxidative stress through nuclear factor(erythroic-derived 2)-like 2 wild-type mice. induction of … WebThe optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user …
WebAnti-Cyp2a5 antibody (catalog number ab3570) was purchased from Abcam (Cam-bridge,MA).Anti–Ppar-g antibody (catalog number sc-7273) was obtained from Santa Cruz Biotechnology (S anta Cruz, CA). Anti-IgG a ntibody was purchased from Cell Signaling Technology (Danvers, MA). Short interfering RNA (siRNA) targeting
WebJan 1, 2015 · A CYP2A5 antibody inhibited 75% of microsomal BR oxidation. The inhibition was absent in control mitoplasts but elevated to 50% after treatment. An adrenodoxin reductase antibody did not inhibit microsomal BR oxidation but inhibited 50% of mitochondrial BR oxidation. Ascorbic acid inhibited 5% and 22% of the reaction in control … can i ask guy out co coworkerWebMar 15, 2024 · As shown in Fig. 1 A, ethanol feeding induced formation of lipid droplets in liver of both Cyp2a5 +/+ mice and Cyp2a5 −/− mice, but the percentage of hepatocytes containing lipid droplets was higher in Cyp2a5 −/− mice than in Cyp2a5 +/+ mice (Fig. 1 B). Consistently, liver TG contents were increased in Cyp2a5 −/− mice to a greater extent … can i ask for time to consider a job offerWebJun 1, 1998 · Immunoblot analysis indicated that CYP2A5 and CYP2G1 are abundant P450 isoforms in OM microsomes. OM microsomal AP and DCBN metabolic activation was … can i ask google to remove a search resultWebMay 15, 2024 · The mechanism of CYP2A5/2A6 increased in different pathogenesis induced liver damage is still unclear. It has been proposed that the common mechanism in the CYP2A5-inducing conditions is a direct or indirect systemic effect elicited by toxicity or tissue damage, rather than the chemical itself (Camus-Randon et al., 1996; Salonpää et al., … fitness centers in portland oregonWebMar 25, 2024 · Western Blot: Cytochrome P450 2A6 Antibody (OTI1D2) - Azide and BSA Free [NBP2-70524] - HEK293T cells were transfected with the pCMV6-ENTRY control … can iask for vacation during my pregnancyWebNov 3, 2024 · Antibodies and biochemical assay kits. Anti-CYP2A5 antibody was a gift from Dr. Risto Juvonen (Department of Pharmacology and Toxicology, University of Kuopio, Kuopio, Finland) and anti-CYP2E1 was a gift from Dr. Jerome Lasker (Hackensack Biomedical Research Institute, Hackensack, NJ). can i ask for salary range before interviewWebJan 1, 2015 · A CYP2A5 antibody inhibited 75% of microsomal BR oxidation. The inhibition was absent in control mitoplasts but elevated to 50% after treatment. An adrenodoxin … can i ask if 意味