Nephrin antibody

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August undefined, 2024

WebAnti-Podocin antibody produced in rabbit (affinity isolated antibody, buffered aqueous solution); Suitable for western blot (chemiluminescent); Anti-Podocin antibody produced in rabbit has been used for Western Blotting and immunohistochemistry; Podocin is a podocyte structural protein, that belo WebValidations: None Available. Applications: WB, IHC and 2 more. Reactivity: Homo sapiens (Human), Mus musculus (House mouse), and Rattus norvegicus (Rat) Nephrin Antibody - BSA Free. NBP1-77303 is a primary antibody from Novus Biologicals.

Phosphorylation of key podocyte proteins and the association with ...

http://www.nephjc.com/news/nephrin-mcd WebAug 7, 2024 · A large part of why nephrin phosphorylation is well characterized compared with other podocyte proteins is because of the existence of several reliable phospho-nephrin antibodies. However, phospho-specific antibodies can be difficult to generate with the specificity needed to reliably detect and quantify the phosphorylation event of interest. dポイント現金払いで貯まる知恵袋

Nephrin in Nephrotic Syndrome — NephJC

WebThe sections were stained using rabbit anti-AGE polyclonal antibody (dilution 1:1,000), rabbit anti-nephrin polyclonal antibody (dilution 1:250), and rabbit anti-caspase-3 polyclonal antibody (dilution 1:250) (90 minutes at RT), which were dissolved in PBS + 3% FBS + 0.25% Triton X-100 for expression of AGE, nephrin, and caspase-3, respectively. WebNephrin Antibodies. (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1)) This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit ... dポイント現在のポイント

Phosphorylation status of nephrin in human membranous …

nephrin Antibody (B-12) SCBT - Santa Cruz Biotechnology

WebAug 12, 2024 · Simultaneously, NEPH1 and NEPHRIN were overexpressed in HEK293 cells and the proteins were immunoprecipitated using their respective antibodies and incubated with purified FYN (500 ng) (set 2). Recombinant SHP-2 protein was then added to the immunoprecipitated complexes (both sets) and the extent of dephosphorylation was … WebApr 14, 2024 · Nephrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti Mouse Nephrin Antigen Affinity purified Polyclonal Antibody (Catalog # AF3159) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557 conjugated AntiGoat IgG Secondary Antibody (yellow; dポイント用途限定先に使うWebRabbit monoclonal (Y17-R) antibody to Nephrin. This antibody recognizes the epitope located between Cys465 - Cys528. WB: 1:5,000, ELISA: 1:50,000-1:100,000. Peptide derived from the centre of the fifth Ig-like domain of human nephrin. Supplied in Tris Buffered Saline, pH 8.0, with 10 mg/ml BSA and 0.05% Sodium Azide. dポイント申し込みキャンペーン

"WebWe generated an antibody to pY1228 in addition to the antibody to pY1208, as the sequence around Y1228 is completely conserved among rats, mice, and humans, and Nck binds to pY1228 [3]. Although it had been uncertain whether Y1228 was the phosphorylation site, we could clearly demonstrate that Y1228 is also a phosphorylation site of nephrin [9]. " - Nephrin antibody

Nephrin antibody

NPHS1 Adhesion Molecule, Nephrin Antibodies

WebSep 1, 2024 · The effect of topiroxostat in anti-nephrin antibody (ANA)-induced podocyte injury. (a) Representative IF findings (upper panel), and the semi-quantitative data of immunostaining (lower panel) of podocyte functional molecules. Nephrin, podocin and podoplanin were observed as a continuous linear pattern along capillary loop in normal … WebNephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized …

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WebNephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin. Packaging. 100 μg. Linkage. The action of this antibody can be blocked using blocking peptide SBP2265. WebAug 3, 2024 · nephrin Antibody (G-8) is a mouse monoclonal IgG 2a κ nephrin antibody, cited in 24 publications, provided at 200 µg/ml. raised against amino acids 23-322 mapping within an extracellular domain of nephrin of rat and human origin. nephrin Antibody (G-8) is recommended for detection of nephrin of mouse, rat and human origin by WB, IP, IF …

WebNephrin not only functions as the core component of the extracellular SD filtration network, ... 1193, and 1217 is the most widely characterized, potentially due to the commercial availability of phospho-specific antibodies for these sites . Phosphorylation at these sites results in the recruitment of Nck, PLC-γ1, ... WebNephrin antibody was raised in guinea pig using a synthetic peptide corresponding to residues 1243-1256 of the intracellular domain of nephrin as the immunogen. Validations. None provided. Applications. Immunohistochemistry (IHC) Immunohistochemistry-paraffin (IHC-P) Western Blotting (WB) Reactivity. Homo sapiens (Human) Mus musculus …

WebAnti-Nephrin (Nphs1) antibody is validated on mouse tissue and recommended for immunofluorescence labeling, IHC, or western blot of materials from rodent and human tissues. Nephrin is encoded by the Nphs1 gene in human. Nephrin is a structural protein making the slit diaphragm in the glomerular podocytes. WebSep 30, 2024 · We report our experience with a C57BL/6 FSGS model induced by polyclonal rabbit anti-mouse nephrin antibody (α-mNep Ab). Methods: α-mNep Ab, which was generated by genetic immunization, was ...

WebThis case strengthens evidence that anti-nephrin antibodies cause disruption of the slit pore diaphragm which appears to be readily responsive to immune therapy. Anti-nephrin mediated podocytopathy may present with a spectrum of glomerular histopathology, which on the background of other susceptibility factors, can lead to more severe presentations …

WebImmunohistochemical analysis of paraffin-embedded mouse kidney tissue using Nephrin Polyclonal Antibody (Product # PA5-20330) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody … dポイント申し込み方法WebNephrin, also known as renal glomerulus-specific cell adhesion receptor and nephrosis 1 (NPHS1) is a 185-200 kDa type I transmembrane protein belonging to the … dポイント用途限定何に使えるWebAug 3, 2024 · nephrin Antibody (G-8) is a mouse monoclonal IgG 2a κ nephrin antibody, cited in 24 publications, provided at 200 µg/ml. raised against amino acids 23-322 … dポイント番号確認 12桁WebNov 14, 2024 · Phosphorylation of nephrin can be induced using FKBP-FRB heterodimers. Nephrin is an essential structural component of the kidney glomerulus, a specialized structure for ultrafiltration of plasma, consisting of eight extracellular immunoglobulin G (IgG) repeats, a transmembrane domain, and an unstructured tail of ∼160 amino acids … d ポイント登録WebNephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin.The immunogen is located within the last 50 amino acids of Nephrin. 纯化方式 Purified by antigen-affinity chromatography 偶联 Unconjugated. … d ポイント登録WebCells stained with primary antibody (green), and isotype control (orange). 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Seems to play a role in the development or function of the kidney glomerular filtration barrier. dポイント登録WebAug 18, 2024 · After red blood cell removal using ammonium chloride potassium lysis buffer and endothelial cell depletion using CD31 antibody (Biolegend, San Diego, CA, USA), nephrin-positive cells were prepared from minced murine kidneys using magnet-activated cell sorting with nephrin antibody (Thermo Fisher Scientific, Waltham, MA, USA). dポイント登録