Siglec-9 fc
WebIn peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T cells (5). It binds equally well to both 2,3- and 2,6-linked sialic acid (5, 6). …
Siglec-9 fc
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WebImmunogen: Human Siglec-9-Fc Application: Flow cytometry (Routinely Tested) Vol. Per Test: 5 µl/test Entrez Gene ID: 27180 Storage Buffer: Aqueous buffered solution … WebMar 8, 2024 · Siglec-9 Fc or control IgG1-Fc at different concentrations was added to the cells. Supernatants were harvested and assayed for IL-17A and IL-10 by ELISA. Intracellular cytokine staining and flow cytometry. Splenocytes or PBMCs were stimulated for 6 h at 37 °C with phorbol 12-myristate 13-acetate (PMA, 50 ng/ml) and ionomycin ...
WebLigands for Siglec-9 are up-regulated in human carcinomas. (A) LS180 (colorectal cancer, CRC) and A549 (NSCLC) cell lines were probed for Siglec-9 ligands using hSiglec-9–Fc chimera (red line ... WebOct 9, 2024 · Aiming to generate soluble Siglecs that are compatible with a variety of assays, four features were incorporated: (1) a His 6 -tag for purification that is directly C …
WebAug 15, 2005 · Siglec-9 ligation induces neutrophil death, which is accelerated by GM-CSF and interferons Since Siglec-9 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), 15, 16 which is usually able to bind and activate inhibitory phosphatases, we hypothesized that Siglec-9 ligation might have inhibitory effects on neutrophil viability. WebIn peripheral blood leukocytes, Siglec-9 is expressed on neutrophils, monocytes, a fraction of NK cells, B cells, and a minor subset of CD8+ T …
Web表达区间及表达系统（Source）. Alexa Fluor 488-Labeled Human Siglec-2, His Tag (SI2-HA2H7) is produced via conjugation of AF488 to Human Siglec-2, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Siglec-2, His Tag is expressed from human 293 cells (HEK293). It contains AA Asp 20 - Arg ...
WebMay 4, 2024 · Siglec-9-Fc bound to SLe x and 6-Su-SLe x (which carries the sulfate on the GlcNAc residue) and more modestly to 6-Su-SLacNAc whereas Siglec-E-Fc bound … commissary montgomery alWebsecreting Siglec-Fc proteins were prepared following standard methods (15). Generation of Monoclonal Antibodies to Siglec-9—Balb/c mice were immunized three times with 10 mg … dswd region 2 addressWebUsing Fc-engineered anti–Siglec-7 and anti–Siglec-9 blocking antibodies, we show reduced tumor burden, thus demonstrating the therapeutic potential of targeting these two Siglecs … commissary nas lemooreWebView our 22 Siglec-9 products for your research including Siglec-9 Primary Antibodies, Simple Plex Assays, Proteins and Enzymes, cDNA Clones, ELISAs, and Proteome … dswd region 3 directorWebAug 22, 2016 · We noted that potentiation of killing by T-Sia versus Tras correlated highly with Siglec-7 ligand abundance (Fig. 5D) and weakly with Siglec-9 ligand abundance (Fig. 5E), as determined by flow cytometry using Siglec-Fc chimeras. Treatment of BT-20 and ZR-75-1 cells—those with the highest expression of Siglec-7 and Siglec-9 surface … commissary nas corpus christiWebJul 31, 2024 · Fluorescence-activated cell sorting (FACS) analyses of primary human tumours revealed robust expression of the CD24 protein in breast cancer cells and ovarian cancer cells, and TAMs from both... dswd region 4 hiringWebApr 23, 2024 · Siglecs (sialic acid–binding immunoglobulin-like lectins) serve in self-recognition and transmit immune inhibitory signals upon binding to a select repertoire of … dswd region 3 directory